Lab 5 Goals and Objectives:
Exercise 13: Negative Staining: Each pair makes slides with:
Staphylococcus aureus (slant) & Bacillus subtilis (slant, 24hr) (Substitute)
Mix both together on same slide. Use Nigrosine NOT India Ink
No smears first: organism in ink
At least one slide-spread slide (Fig 13.2)
Oral organisms optional
Exercise 14: Capsular Staining: Use 11th edition instructions in supp. packet!!!
Each pair makes slides with:
Klebsiella pneumoniae (broth)
Klebsiella pneumoniae (slant) (additional culture) On separate slides.
Use Nigrosine NOT India Ink, No smears first: organism in ink
Minimum one slide each of slant and broth (Fig 14.1 in 11th edition)
Exercise 16: Spore Staining
Each pair make at least 2 separate slides with:
Bacillus subtilis (slant, 36hr)
Clostridium sporogenes (slant, 4 day)
Schaeffer-Fulton Method only (Fig 16.2)
Make all smears first so they can dry before heat fixing!!!
Split the work to save time: make sure everyone sees all.
Figure 13.2
Negative Staining
No smear first: mix organisms in ink, spread, and dry
Do NOT touch
dropper to slide
On Website
Fig 14.1 pg 103 from 11th ed.
Capsular Staining
Do NOT touch
dropper to slide
nigrosin
paper towel
Figure 16.2
Endospore Staining
Make heat fixed smear slide first!
Steam tray
No paper towel
paper towel
Lab 5 Goals and Objectives:
Exercise 13: Negative Staining: Each pair makes slides with:
Staphylococcus aureus (slant) & Bacillus subtilis (slant, 24hr) (Substitute)
Mix both together on same slide. Use Nigrosine NOT India Ink
No smears first: organism in ink
At least one slide-spread slide (Fig 13.2)
Oral organisms optional
Exercise 14: Capsular Staining: Use 11th edition instructions in supp. packet!!!
Each pair makes slides with:
Klebsiella pneumoniae (broth)
Klebsiella pneumoniae (slant) (additional culture) On separate slides.
Use Nigrosine NOT India Ink, No smears first: organism in ink
Minimum one slide each of slant and broth (Fig 14.1 in 11th edition)
Exercise 16: Spore Staining
Each pair make at least 2 separate slides with:
Bacillus subtilis (slant, 36hr)
Clostridium sporogenes (slant, 4 day)
Schaeffer-Fulton Method only (Fig 16.2)
Make all smears first so they can dry before heat fixing!!!
Split the work to save time: make sure everyone sees all.