Biotechnology Review
The test is 60 points – 11 multiple choice and 11 short answer (worth 49 points). There will be 5 bonus points available.
Topics
 Selective Breeding
o Definition
o Give two types of selective breeding
o List pros and cons of each type
 Cloning
o Definition
o Compare / Contrast plant and animal cloning
o Give two types of animal cloning
o What ethical issues are associated with cloning
 Restriction Enzymes
o Source (where did they come from?) and original purpose
o Genetic Engineering use (what do we use them for and why)
o How do they work
o What do they produce
 Transformation
o Definintion
o Genetic Engineering use ( why do we do this)
o Explain the process of producing recombinant plasmids and incorporating them into bacteria so that the
bacteria express foreign DNA
 Clone Library
o Definition
o How do we do this
o Why do we do this
 Complementary DNA
o Definition
o How is it produced
o How is different from normal genomic DNA
 Transgenic / Genetically Modified Organisms
o Definition
o Give examples of bacteria, plants and animals – explain modification, benefit, and concern
 Nucleic Acid Probe
o Definition
o Purpose
 Micro Array
o Definition
o Purpose
 Gel Electrophoresis
o Definition
o Purpose
o Process
o Potential applications
 Gene Therapy
o Definition
o Purpose
 PCR
o Definition
o Purpose
o Process
 Human Genome Project
o Definition
o Purpose
o Results / Outcome