DNA Replication, Transcription, Translation, and Mutation Worksheet
Cystic Fibrosis Gene: When both copies of this gene are mutant, individuals have lung and
kidney problems.
You will be able to write DNA sequence for the complementary strand (1).
You will be able to write the RNA and protein sequence (2 & 3).
You will be able to note the consequence of each DNA mutation for the protein.
A. Normal Gene (a small portion only)
5'- acc att aaa gaa aat atc atc ttt ggt gtt tcc tat gat - 3'
1.
2.
3.
B. Known Mutant Gene
5' - acc att aaa gaa aat atc atc --- ggt gtt tcc tat gat - 3'
1.
2.
3.
C. Hypothetical Mutant Gene 1
5' - acc att aaa gaa aaa atc atc ttt ggt gtt tcc tat gat- 3'
1.
2.
3.
D. Hypothetical Mutant Gene 2
5' - acc att aaa gaa caa tat cat ctt tgg tgt ttc cta tga t - 3'
1.
2.
3.
Mutation - a change in the DNA
Point mutations - when a single nitrogen base is deleted, inserted or substituted.
Frameshift Mutation- when many amino acids coded for are different because of an
insertion or deletion.
The following example shows how these 3 kinds of mutations work. Fill out the RNA
and protein for each and compare the mutations to the original. The mutation is in bold.
Original DNA T A C G C G T G C A C G A T G C A G T A G T A C
RNA
Protein
Mutation 1 DNA T A C G C G T G C A C G A T C C A G T A G T A C
RNA
Protein
Type of Mutation:
Mutation 2 DNA T A C G C G T G C T C G A T G C A G T A G T A C
RNA
Protein
Type of Mutation:
Mutation 3 DNA T A C G C G C T G C A C G A T G C A G T A G T A C
RNA
Protein
Type of Mutation: