Boston University Department of Biology
Dr. Elizabeth Godrick
BI 114 LAB SCHEDULE - FALL 2009
Laboratories meet 2 times per week for 1 1/2 hours each
TF:
Office:
Office Hours:
Email:
Sections: B1 MW 3-4:30pm;
C1: TR 9:30-11 am; C2: TR 2-3:30 pm ; C3 TR 3:30-5:00pm
D1: WF 10:30-12 pm; D2 WF 1-2:30pm
Laboratory Manual: H. J. Benson, Short Version (11th Ed.)
Materials needed: Materials needed: Laboratory- notebook (SCI 301) and disposable latex gloves
Lab policies:
Read the exercises in the Benson’s Lab Manual PRIOR to class
NO make up Labs or Exams
If you must miss a section ask for approval to attend a section on a Monday or Wednesday
NO open toed shoes
NO food, drinks (coffee and water bottles included), gum, cell phones, audio equipment
Late assignments will NOT be accepted
Attendance will be taken before every class.
Lab #
Date
Laboratory
1
Week I
______
a)
b)
c)
d)
2
Week I
______
a) Data Review: A Synthetic Epidemic Ex 60
b) Use and care of the microscope Ex 1, 4
c) Prepared Slides (mixture of 3 shapes), Letter “e”
d) Ubiquity of Bacteria Ex. 6
3
Week II
____
a) Data Review: Ubiquity of Bacteria Ex 6
b) Aseptic Technique Ex 8
c) Preparation of a smear Ex 10
d) Gram stain Ex 14
4
Week II
____
a) Data Review: Aseptic Techniques Ex 8
b) Spore stain Ex 15
c) Morphology of Fungi/Molds Ex 7 (Demo)
5
Week III
____
a)
b)
c)
Alcohol Evaluation Ex 32
Motility Determination Ex 17 Tube Stab and Hanging Drop
Video of Experimental Design for Humans
6
Week III
____
a)
b)
c)
Data Review: Alcohol Evaluation Ex. 32
Data Review: Motility Determination Ex 17
Acid Fast staining procedure Ex 16
Introduction to lab
Safety procedures; general information; Use of Bunsen Burner
Info on bacterial growth: Media/Temperature
A Synthetic Epidemic Ex 60
7
Week IV
____
a)
b)
c)
d)
8
Week IV
____
a) QUIZ I
b) Data Review: Ultraviolet Light: Lethal Effects Ex 30
c) Data Review: Effectiveness of Hand Scrubbing Ex 35
d) Pure Culture Techniques continued (subculture)
e) Demonstration of physical agents, shaker, stirrer, pipette men,
spectrophotometer, filtration, autoclave, hot oven, laminar
flow hood, warm room, ultraviolet light.
9
Week V
____
a)
b)
c)
Culture Characteristics Ex 28 & 29 (pH and osmotic pressure)
Pure Culture techniques final lab (Check for pure cultures)
Culture Media: Selective, Differential, and Enriched. Ex. 18
(Explanation)
10
Week V
a)
b)
Temperature effects on bacteria Ex 27, 26
Data Review: Cultural Characteristics Ex 28 and 29
11
Week VI
No lab
12
Week VI
____
a)
b)
c)
d)
e)
Data Review: Temperature effects on bacteria Ex 27, 26
Morphological Study of Unknown Overview Ex 36
Use of Bergey’s Manual Ex 41
Hand out unknown cultures: Quadrant Streak
Enumeration of Bacteria Ex 20
13
Week VII
____
a)
b)
c)
Data Review: Enumeration of Bacteria ex 20
Morphological Study of Unknown
Inoculation of Enterotube Ex 43
Cultivation of Anaerobes Ex 19
a)
b)
c)
d)
Study normal flora of GI Tract (Godrick notebook)
Data Review: Inoculation of Enterotube Ex 43
Data Review: Cultivation of Anaerobes Ex 19
Gram Stain of Unknown
14
15
Week VII
Lab Report
Due
Week VIII
a)
____
Pure Culture Techniques Ex 9 (Quad Streak)
Ultraviolet Light: Lethal Effects Ex 30
Effectiveness of Hand Scrubbing Ex 35
How to write a scientific paper Appendix VII (Godrick)
Physiological characteristics Ex 38
Catalase, glucose, lactose, oxidase and Citrate Tests
Reductase test Ex 49
16
Week VIII
a)
b)
c)
Bacterial count in food Ex 46
Microbial spoilage of canned food. Ex 50
Morphological Study of Unknown: Preliminary Staining
a)
b)
c)
d)
Data Review: Bacterial count in food Ex 46
Data Review: Microbial spoilage of canned food Ex 50
Bacterial Examination of water Presumptive Test Ex 47
Morphological Study of Unknown
Week IX
____
a)
b)
c)
QUIZ II
Bacterial Examination of water Completed Test Ex 47
Morphological Study of Unknown
19
Week X
____
a)
b)
c)
Bacterial Examination of water Confirmed Test Ex 47
Morphological Study of Unknown
Antimicrobic sensitivity testing Ex 33
20
Week X
____
a)
b)
c)
Data Review: Antimicrobic sensitivity testing Ex 33
Simulated ABO;RH Blood Typing (Godrick notebook)
Completion of Morphological Study of Unknown
21
Week XI
____
a)
ELISA test (Godrick notebook)
22
Week XI
Final LAB Exam
23
Week XII
Morphological Study Oral Presentations and Written Reports
17
Week IX
_____
18
Laboratory Reports (5,15)
Laboratory Notebook
Quiz I
Quiz 2
Final Laboratory Exam
Oral
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20 pts Laboratory Instructor will check your laboratory notebook
15
for concurrent laboratory work.
20
20
20
5
100 --Laboratory grade is 30% of total course grade.
Notice
If you have any kind of chronic health disorder, a letter from your doctor is required
for participation in the laboratory. This letter should state that your exposure to normal
risks in an undergraduate microbiological laboratory is acceptable for you.
After you have reviewed the laboratory syllabus/manual with your physician, please give
the letter to your laboratory instructor.
The author would like to thank Jan Blom for his excellent contributions, both
academically and administratively, toward the presentation of these laboratories.