Micro module 4 exam practice questions 1. True or false differential media is best suited for distinguishing between two similar species of bacteria. Growth media does not contain restrictive factors, while selective media is best used to encourage the growth of one microbe while simultaneously discouraging the growth of the other. Since 2 similar species of microbes are being studied, they must be differentiated under similar but just slightly different conditions (Differential media) 2. A researcher is asked to determine which of two vials contains E coli and which contains Salmonella. Knowing both are Gram negative, while only one of them is capable of fermenting lactose, which type of media would best be suited? A. Growth Media B. Selective media C. Differential media distinguishes between two, often related, microbes. D. Non-selective and non-differential media 3. What are the requirements of a fastidious microbe? A fastidious microbe is an Organism with complex growth requirements such that if absent it will not grow. Enriched medias thus contain these specific and essential nutrients required for the growth of a particular subset of microorganisms. 4. True or false? LB Agar is classified as a selective non differential media. LB Agar is the most basic type of Agar and like LB media, supports the growth of virtually all microbes without restriction. 5. What is Agar used for in microbiology? Agar is used to create a solid, smooth surface on which microbes can grow. 6. Blood Agar is which type of medium? Select all that apply: A. Differential- Blood Agar can be used to differential between species. Based-hemolytic activity. B. Selective and differential C. Enriched D. Selective 7. Match the following hemolytic Class with its description of activity. 1. Alpha hemolysis – Greenish-brown color- Incomplete hemolytic activity 2. Beta hemolysis - Distinct zone of clearing- Complete hemolytic activity 3. Gamma hemolysis – No change- No hemolytic activity 8. Columbia CNA Agar is used to isolate. A. Gram positive- CNA Agar restricts gram (-) microbes. Only gram (+) microbes will grow. B. Mycobacteria C. Gram negative D. Gram positive and gram negative 9. True or false? Chocolate is not a component of chocolate Agar plates. The name is derived based on the color that comes from the presence of cooked, red blood cells in media. 10. An unknown microbe is streaked onto it MacConkey Agar plate. After an overnight incubation at 37°C, growth is observed. Would a gram stain be necessary? Why or why not? No, a gram stain would not be necessary, as only gram (-) microbes will grow on MacConkey agar. 11. In an attempt to detect the presence of the pathogenic strain of E coli 0157:H7. A researcher spread a culture onto the MacConkey Agar with failed results. What type of Agar should they correctly try next? Why? The microbe should be plated on SMAC (Sorbitol MacConkey Agar.) As it is specifically formulated to detect 0157:H7. Pathogenic E coli cannot ferment sorbitol while Non-pathogenic E coli can ferment both sorbitol and lactose. Therefore, colonies that ferment (acidic conditions; non-pathogenic) can be differentiated from non-fermenters (neutral to basic conditions; pathogenic.) 12. In Eosin Methylene Blue (EMB) agar, What color indicates the presence of E coli? Metallic green. 13. Mannitol salt Agar will turn what color in the presence of the pathogenic strain Staphylococcus aureus? Yellow pathogenic staph aureus will turn the Agar from red to yellow. 14. What is the process of spreading a bacterial culture onto a Petri dish called? Plating. 15. Describe the primary advantage of using a Petri dish over growing a liquid culture. The primary advantage is that cells are held into place. When grown in a nutrient broth, bacterial cells can multiply but are free to move around in solution. When grown on Agar within a Petri dish, they are fixed In such a way as to form colonies. 16. True or false? The Visualization of colonies on a Petri dish represents bacterial cells that have often multiplied a million times over. To form a bacterial colony, the initial cell must have multiplied many times, often > 1 mil for the eye to see it. 17. True or false? The purpose of a quadrant streak is to expand a bacterial population. Quadrant streak purpose is to make individual colonies so a single pure bacterial sample can be isolated. 18. To be considered a pure culture, the sample (1) can be tracked back to a single cell and (2)_____. The culture must be free from external contaminants. 19. A dilution gradient is formed when carrying out what generalized plating strategy? A quadrant streak (phase dilution) 20. And what phase of dilution streak would you expect to find the lowest concentration of bacteria like P2 or P4?The phases rank from highest concentration to lowest. P1 > P2 > P3 > P4. 21. True or false: when performing a Dilution streak. A new or sterilized loop is not required for each phase as long as the bacterial culture is pure A new or sterilized loop is absolutely required for each phase. Failure to do so would prevent the establishment of a dilution gradient, as the same bacterial concentration would be spread across both phase regions, regardless of whether or not the culture is pure. 22. In order to encourage growth of a slow growing microbe, what might a researcher do during a phase dilution streak? A researcher may either opt to perform only a three phase dilution streak or pass the loop through the previous phase multiple times (As opposed to only once). 23. True or false? To restrict the growth of a pathogenic microbe, a researcher might decrease an incubator from 37°C to 25°C. Pathogenic strains of bacteria tend to grow faster than non-pathogenic strains at 37°C. Researchers may set incubators at 25°C to restrict its growth. 24. When given an unknown bacterial sample, the first step is to expand the current bacterial population. Which form of media best suits its needs and why? 1. MSA Agar 2. LB Media. All other options are all forms of selective media, meaning they may potentially inhibit the growth of the unknown sample. The culture should be first expanded and then placed onto selective/differential Agar plates. 3. MacConkey Agar 4. Columbia CNA Agar. Exam questions from- Portage micro 171 module 4- DOUBLE CHECK THESE ANSWERS 2. A researcher is asked to determine if a sample contains Neisseria meningitides. Knowing Neisseria meningitides is slow growing and other foreign microbes may also be present in the culture, which type of media would be best suited? A. Selective media- If additional foreign microbes may be present in your sample and you know Neisseria meningitides is slow growing, you must restrict the unwanted microbes while Supporting the growth of Neisseria meningitides. Thus, you are selectively encouraging one microbes growth, while selectively restricting others. B. Selective and Differential media C. Growth media D. Differential media 8. Columbia CNA agar is most closely related to which media: A. Trypticase Soy Agar B. MacConkey Agar C. Blood agar- CNA agar is similar to BAP as it is also enriched with blood and allows for differentiation based on hemolytic patterns D. EMB agar 9. True or False: Chocolate agar gets its brown color from cocoa to produce an enriched media. Chocolate is never added to the media. The name is derived based on the color that comes from the presence of ‘cooked’ (lysed) red blood cells in the media 10. A researcher is studying a strain of E. coli currently growing on a MacConkey plate. However, the researcher can’t remember if E. coli is Gram-positive or Gram-negative. Would a Gram stain be necessary to confirm? Why or why not? No. A Gram stain would not be necessary, as only Gram-Negative microbes will grow on MacConkey agar. Thus, E. coli is a Gram-Negative microbe 12. What is the Gram status (positive or negative) of microbes growing on Eosin Methylene Blue (EMB) agar plates? Gram-Negative. EMB plates specifically restrict the growth of Gram-Positive bacteria. 13. Which type of agar media is best suited to support the growth of Staphylococcus? A. Mannitol salt agar B. Columbia CNA agar C. Blood agar D. Chocolate agar E. MacConkey agar 16. True or False: The visualization of colonies on a petri dish represents bacterial cells that have often multiplied a million times over. 18. Which of the following are correct in order to be considered a pure culture? Select all that apply. Obtained by pour plate serial dilution method Obtained by a 4-phase dilution streak method Obtained by a serial dilution method Free from contaminants Single organism Long lifespan Multiple species 20. In what phase of a dilution streak would you expect to find the highest concentration of bacteria, P2 or P4? Highest concentration would be P2 21. True or False. When performing a dilution streak a new (or sterilized) loop must be used for each phase. Failure to do so would prevent the establishment of a dilution gradient, as the same bacterial concentration would be spread across both phase regions. 22. What might a researcher do to restrict growth of a microbe with a short generation time that has already been streaked on an agar plate? Decrease incubation time OR incubate at a different (lower) temperature 23. True or False. Pathogenic strains of bacteria tend to grow slower than normal non-pathogenic bacterial strains. Exam 4 – Practice questions 3- All exams-N/a 1. True or False: Growth media is best suited for distinguishing between two similar species of bacteria. Growth media is designed to simply support (and not restrict) microbial growth. 2. In order to visual individual colonies of bacteria would you culture your sample in a liquid media or on a solid (agar) media? Why? Solid (agar) media. The primary advantage is that cells are held into place. When grown in a nutrient broth, bacterial cells can multiply but are free to move around in solution. When grown on agar within a petri dish the fixed in such as way as to form colonies. 3. When performing a quadrant streak, the sample is spread across the plate in such a way as to form what? A dilution gradient is formed. The resulting gradient should always contain within it the growth of individual colonies. Question 1 Identify the following disease A. Tetanus B. Anthrax —the distinct black center at the site of infection is a hallmark of anthrax infection C. Gas gangrene D. Frost bite Question 2 A 5-month-old child is given honey at breakfast and begins showing symptoms consistent with botulism. Would the child’s illness be classified as foodborne, infant, or wound botulism? Infant. Although food (honey) is consumed, foodborne botulism is derived from improperly canned, low acid foods under anaerobic conditions. Such conditions were not described. No puncture wounds or injuries were described, ruling out wound. The child is suffering from infant botulism, as honey is a potential carrier of clostridium. The intestinal track of young children is still developing and clostridium is able to colonize and produce the associated toxin. Question 3 Identify the medical condition characterized by robust carbohydrate fermentation under anaerobic conditions, swelling of the infected areas and fever. Gas gangrene. The key identifier here is the robust carbohydrate fermentation, which manifests as intense gas production and swelling—both trademark conditions of gas gangrene Question 4 Which form of the plague is the rarest? What does it target? Septicemic plague is the rarest and it targets the blood system. Question 5 The concept of treating all samples, whether known or unknown, as potentially hazardous (or pathogenic) materials is known as __________. Universal precautions Question 6 List at least 3 observations a researcher would be sure to note while assessing an unknown microbial sample. A lab researcher would be certain to note: 1. Size and shape 2. Any observable motility 3. Gram status (positive or negative) 4. The presence of any chemical reactions 5. Changes in color localized to the organism or the surrounding media 6. Capture (or draw) images of any of the characteristics described above Question 7 While observing an unknown sample of limited amounts, a researcher must determine the following observations: (1) the presence of any motility and (2) its Gram status using the same sample—the liquid sample cannot be divided. Which would you determine first and why? You must determine motility before determining the Gram status. Motility requires a wet mount, while Gram staining requires heat fixing the sample. If one were to begin with the Gram stain the heat fixation process would kill the organism, making any observations regarding motility impossible. The correct approach would be to place the liquid culture on a glass slide and determine its motility status. Next, the same liquid culture can be heat fixed and Gram stained. Question 8 A facultative anaerobe is a microorganism capable of growth under what conditions? A facultative anaerobe is capable of growth under aerobic (with oxygen) and anaerobic (without oxygen) conditions. Question 9 As Streptococcus is catalase negative would it thrive or die in the presence of peroxides? Why? Streptococcus would not survive in the presence of peroxides—it is unable to breakdown peroxides (catalase negative). Left unchecked, peroxides would damage the cellular integrity of Strep causing lysis/cell death. Question 10 Streptococcus is most often streaked onto: Blood agar plates to determine its hemolytic properties, which aids in the classification (and differentiation) process. EMB agar Spirit Blue agar Chocolate agar Question 11 The distinctions for Lancefield subgroupings lie in its: (select all that apply) Catalase activity Carbohydrate composition of antigens (present in the cell wall determines the Lancefield groupings) ALL strep under Lancefield groupings are catalase negative and beta hemolytic. Hemolytic activity All of the above Question 12 Left untreated, strep throat can progress to ______ , which displays _____ hemolytic activity. Rheumatic fever; beta Strep throat (also known as strep. Pharyngitis) can lead to Rheumatic fever if left untreated. Strep. Pharyngitis displays beta-hemolytic activity. Strep. Pharyngitis; beta Strep. Septicemia; gamma Strep. Pharyngitis; alpha Question 13 When a bacterium neither harms nor benefits from the host from which it obtains nutrients, it is referred to as being ___________. Commensal Question 14 Syphilis is caused by: Gram-Negative spirochete Question 15 The alpha-toxin perfringolysin is associated with which medical condition caused by Gram-positive anaerobic bacteria? Gas Gangrene Tetanus Leprosy Botulism Question 16 Match the following diseases with their respective symptoms if left untreated: 1.Gonorrhea - Cardiac and neurological complications 2. Syphilis -- Paralysis, blindness and dementia 3. Chlamydia -- Infertility and pelvic inflammatory disease. Question 17 What causes these? 1. Painfully swollen lymph nodes- Bubonic plague 2. Respiratory failure associated with lock-jaw- Tetanus 3. Sore throat, ocular discharge and fever- Conjunctivitis Question 18 Define the concept of universal precautions. Universal precautions means any and all samples, whether known or unknown, are to be treated as potentially hazardous (or pathogenic) materials. Question 19 Identify the disease based on the following (select all that apply): It is a small, acid-fast rod usually remaining asymptomatic for up to 20 years. It affects the skin, nerves, upper respiratory tract and eyes of infected individuals. A. Tuberculosis B. Leprosy is characterized by its ability to remain dormant for up to 20 years as well as the effects observed relative to the patients skin, nerves, lungs and eyes. C. Scalded-skin syndrome D. Hansen’s disease- Another name for Leprosy. E. Bacterial conjunctivitis Question 20 Define commensal bacteria. The bacterium neither harms nor benefits from the host from which it obtains nutrients. Question 21 How can Staphylococcus be differentially tested? Staphylococcus species are capable of growth in the presence of bile salts Question 22 Match the following symptoms with their respective diseases: 1. Folliculitis- Pus-filled lesions on skin or hair 2. Scalded-skin syndrome- Ruptured pustules; treated with Penicillin 3. Impetigo- Childhood skin disease near mouth/nose 4. Conjunctivitis- Infection of thin, transparent scleral tissue 5. Ophthalmia Neonatorum- Infection occurs at time of birth Question 23 While visiting his grandparents, a 10-year-old child has green beans for dinner that were harvested from his grandparents’ garden and canned the previous year. Severe illness sets in, and the child is taken to the hospital. Botulism is suspected, but what form? Explain your answer. Foodborne botulism. The child is too old (> 6 months) to be considered for infant botulism and wounds were not present. The green beans were likely improperly canned and under the low-acidic, anaerobic conditions, bacterial growth was encouraged. Question 24 Describe the symptoms and treatment regimen for someone infected with Clostridium perfringens: Clostridium perfringens (also known as Gas gangrene) presents with muscle necrosis, swelling of infected areas, fever and intense gas production. Treatment entails removal of all infected tissue, often resulting in amputation of the affected areas, in conjunction with heavy antibiotic therapies. Question 25 Why does the alpha toxin (perfringolysin), produced during an infection of gas gangrene, cause cell death? The toxin perforates the membrane. It forms pores in the plasma membrane of host cells resulting in uncontrolled ion fluxes and eventually cell lysis and death. Question 26 You develop a fever, chills and pneumonia after recently using a warm midst vaporizer in an attempt to open your sinuses. What bacterial disease would a doctor suspect is causing your symptoms? Would the doctor suspect you contracted it from touching (potentially) contaminated doorknobs? Legionnaires. No—legionella cannot be spread simply by direct contact between people. Legionella can only be transmitted through droplets small enough to be breathed in. Question 27 Which form of the plague is highly virulent? What does it target? Pneumonic plague is highly virulent and targets the respiratory (lungs) system. Question 28 Rheumatic fever displays ______ hemolytic activity and occurs when _______ is left untreated. Beta; Strep. Pharyngitis Strep throat (also known as strep. Pharyngitis) can lead to Rheumatic fever if left untreated and displays beta-hemolytic activity. X Beta; Rheumatic fever X Alpha; Strep. Pharyngitis X Gamma; Strep. Septicemia Question 29 Gonorrhea is caused by: A. Diplococcic bacteria Question 30 Match the step of infection to its description. Invasion - Pathogen passes through or between cells to access deeper tissues and nutrients Evasion - Pathogen suppresses host’s immune system so it can continue replicating Transmission - Pathogen is passed to another host to restart lifecycle Question 31 True or False. An acid-fast stain is best suited to identify tuberculosis (as opposed to a Gram stain). TB shows a poor Gram stain and should be screened via an acid-fast stain. Question 32 True or False. Although TB must be inhaled and reside in the lung in order to cause infection, TB can also spread to the brain, spine, and kidneys. TB can move systemically through the blood to infect other areas (brain, spine and kidney). However, when located outside of the lung, TB tends to be non infectious Question 33 True or False. There are currently no cures for tetanus. Although the symptoms can be treated it is only to manage the discomfort not to cure. Question 34 True or False. Pneumonic plague is often characterized by painfully swollen lymph nodes. Pneumonic plague targets the respiratory system while bubonic targets the lymph system. Question 35 True or False. The Lancefield groups are used to subdivide antigenic groups of gamma-hemolytic Streptococcus. The Lancefield groupings are used to subdivide beta-hemolytic Strep. Question 36 True or False. Under most circumstances, staphylococcus can be found in 30% of human population where it remains pathogenic. Question 37 True or False. Chlamydia trachomatis can be grown on an agar plate alone. Chlamydia is an obligate parasite and requires a host (viable cells) for its growth. Question 38 True or False. Staphylococcus is the only causative agent of conjunctivitis. There are bacterial (staph) and viral forms of conjunctivitis, both resulting in the inflammation of the conjunctiva and ‘pink eye.’ Question 39 True or False. Directly ingesting clostridium or its associated spores will not cause illness in adults Question 40 True or False. The Lancefield groups are used to subdivide antigenic groups of alpha-hemolytic Streptococcus. The Lancefield groupings are used to subdivide beta-hemolytic Strep. Question 41 True or False. Under most circumstances, staphylococcus can be found in ~80% of human population where it remains non-symptomatic. Staphylococcus in only found (non-symptomatic) in ~30% of the human population. Question 42 True or False. Although there is no cure for tetanus, it can be prevented through vaccination Question 43 True or False. The causative agent of conjunctivitis can be either bacterial or viral. There are bacterial (staph) and viral forms of conjunctivitis, both resulting in the inflammation of the conjunctiva and ‘pink eye.’ Question 44 True or False. Tuberculosis is best identified by a Gram stain. Question 45 True or False. When located outside of the lung, TB is usually non-infectious. Question 46 True or False. Under most circumstances, staphylococcus can be found in ~80% of human population where it remains non-symptomatic. Staphylococcus in only found (non-symptomatic) in ~30% of the human population. Question 47 True or False. The causative agent of conjunctivitis can be either bacterial or viral. Question 48 True or False. Tuberculosis is best identified by a Gram stain. Mycobacterium show poor Gram staining and w/an acid-fast stain. Question 49 True or False. You can become infected with TB simply by sharing a drink with someone who is currently infected with TB. Question 50 True or false: Only directly ingesting the pre-formed Clostridium toxin (neither the bacteria nor its spores) will cause disease in adults. The mature (adult) microbiota is capable of killing the bacteria/spores. Question 51 True or False. Bubonic plague is often characterized by painfully swollen lymph nodes. Question 52 True or False: The Lancefield groupings are used to subdivide beta-hemolytic Strep. Question 23 True or False: Septicemic plague targets the respiratory system True or False: Septicemic plague targets the blood True or False: True or False: True or False: True or False: True or False: True or False: True or False: True or False: True or False: True or False: True or False: True or False: True or False: Can viruses be classified as either prokaryotic or eukaryotic microorganisms? No, they are considered obligate intracellular parasites No. Viruses are neither prokaryotic nor eukaryotic What is an obligate intracellular parasite? Your Answer: Obligate intracellular parasite can't replicate on their own and need a living host As an obligate intracellular parasite, viruses cannot (obligate) replicate unless inside (intracellular) a living host cell. As a parasite, a virus depends on its living host to provide the framework (proteins, etc) to replicate—to make additional copies of itself. What is a viral capsid? Your Answer: A protective structure, similar to a membrane, containing genetic material The capsid is a membrane-like protective structure that contains the genetic material, similar to the nucleus of a eukaryotic cell. What is the viral envelope, and what does the presence (or absence) of it tell you about the type of virus? Your Answer: A viral envelope is an additional membrane taken from the host cell membrane, that surrounds the capsid. The presence or absence can help to distinguish it is an animal virus (usually enveloped) or a plant/bacteria (non-enveloped) infecting virus. The envelope surrounds the viral capsid, is derived from the host cell membrane and serves as an additional barrier to the external environment. The overwhelming majority of animal viruses are enveloped whereas the majority of plant or bacteria-infecting viruses are not What type of microscope would be used to study the shape of a viral capsid? Your Answer: Electron microscope How does the viral envelope both aid in cell entry as well as hide it from being detected by the immune system? Your Answer: The envelope is copied from the host cell membrane, which hides the virus. Now the virus appears to be a normal host cell, this helps it evade detection from the immune response. The proteins on the virus find and link to compatible proteins on the host cell, which then fuses with the membrane to gain entry The viral envelope contains the necessary proteins required for interaction with host cells. As the envelope is derived from the host cell membrane, this camouflages the virus and lowers the likelihood of immune detection by making it appear similar to the normal biological host cell What must happen before viral replication of its genome can occur? Your Answer: Attachment and entry What is a bacteriophage? Your Answer: Type of virus that infects bacteria What structural aspects make bacteriophages distinct from viruses that infect either animals or plants? Your Answer: 20 sided polygon capsid head group that contain viral genomes with a helical tail that have tail fibers (extensions) that help bind it to the host. They do not pass their capsid into host cell, but the helical tail penetrates and shoots the viral genome through the tail and into the cytoplasm. Infections have not been found in plants or animals. Bacteriophages possess an icosahedral capsid head group and a helical tail. Attached to the tail are fibrous extensions or ‘legs’ that aid in binding host cells. Notably, this particular structural arrangement has not been observed among viruses that infect either animals or plants. Which viral replicative process results in the destruction of the host cell? Your Answer: Lytic replication The lytic replication cycle occurs when a bacteriophage replicates within a host until it ruptures, effectively destroying the host bacterial cell. The phrase lytic stems from ‘lysis’, meaning to rupture or disintegrate (breakdown) a cell Describe the appearance of a bacterial culture infected with virus at the time of infection versus later once viral replication is occurring at a high rate. Your Answer: At the time of infection there is high degree of turbidity (cloudiness). As the lytic cycle precedes the more the bacterial cells die off. As they do, the media starts and continues to turn get clear During the initial inoculation of virus and bacteria, the media has a high degree of turbidity (cloudiness). However, as the lytic cycle progresses and more and more bacterial cells are destroyed, the culture media turns from turbid to clear, as turbidity is a function of the number of intact bacterial cells present in the media. Thus, as bacterial cell numbers decrease due to lysis, the media becomes clearer. To what family of viruses does rubella come from and how is it transmitted? Your Answer: Togaviridae, transmitted by airborne particles Rubella virus belongs to a family of viruses called Togaviridae. Rubella is transmitted via airborne particles such as when a person coughs. What is the common name for rubeola, and is it a DNA or RNA virus? Your Answer: Measles and it is an RNA virus Rubeola is commonly referred to as the measles and belongs to the family of viruses called Paramyxovirus. Rubeola viruses are RNA viruses with a genome ~ 15,000 nucleotides long. What disease state emerges as a viral reactivation of a prior infection with the varicella virus? Your Answer: Shingles, it is a latent virus that lies dormant until activated. Causing painful, but localized, blisters Shingles, also known as Herpes zoster, is due to the reactivation of the varicella-zoster virus (VZV). Because VZV lies dormant in inactive nerve cells, its reactivation typically results in painful blisters. However, unlike the initial chickenpox rash, the blisters are localized and limited to small areas What linear, double-stranded, enveloped DNA virus is the first virus (as of October 26, 1977) to be declared eradicated? Your Answer: Smallpox Although once a major cause of death in the world, a smallpox vaccine was developed in 1796 by Edward Jenner. Global vaccination efforts have prevented smallpox from appearing in humans since October 26, 1977, making smallpox the first infectious disease ever to be eradicated. What are the two key glycoproteins found in the influenza virus? Your Answer: Hemagglutinins and neuraminidase The viral envelope contains two key glycoproteins, hemagglutinins (HA) and neuraminidase (N). Hemagglutinins are heavily involved in the entry of the viral particle into a host cell, while neuraminidase proteins are involved in the budding and release of new viral particles from the host cell. Describe the composition of HIV. Your Answer: HIV contains two copies of single-stranded, linear RNA that is enclosed by a cone shaped capsid that surrounds RNA strands and is Made up of 2,000 copies of the viral protein p24. A viral envelope surrounds the capsid. Within this envelope are two glycoproteins, gp120 and gp41, which are needed to bind and make entry into the host cell HIV contains two copies of single-stranded, linear RNA that is enclosed by a capsid. The conical capsid surrounds the two RNA strands and is comprised of ~2,000 copies of the viral protein p24. A viral envelope derived from the cell host membrane then surrounds the viral capsid. Within this envelope are two key glycoproteins, gp120 and gp41, which are essential for viral binding and entry into the host cell. NEW Giemsa staining is used to detect the following except. Gonorrhea
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