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DNA Diagram Project
Each ofthe following three diagrams is based on the following DNA
sequence
Only one strand of the double helix is shown
3prime -TATATACCTACGTACAACGTACTTTTACGTCCCGAATATA- 5 prime
General Directions:
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Each of these three diagrams must be "created"/ these are not diagrams you can copy from a source
Quality / neatly done work is expected .
Carefully plan each diagram before you begin
Each diagram has its own set of directions - follow those directions carefully
Diagrams 2 & 3 show a "frozen moment in time" for a dynamic, moving process. The more you
add in the way of arrows, labels, annotations, highlighting, etc., the clearer your diagram will
become.
 Consider using color coding / highlighting to show differences in strands, RNA vs. DNA
nucleotides, etc.
Diagram 1: DNA structure
 Use the first eight nucleotides only (16 total) in an antiparallel ladder diagram
 Each nucleotide: show atom by atom phosphate groups, deoxyribose numbered
carbon
backbone, named nitrogenous bases labeled as purine (pur) or pyrimidine (pyr)
 Show correct number of hydrogen bonds (as dotted lines) between complementary
bases
 Clearly label the 5' and 3' ends of each of the strands
Diagram 2: DNA Replication
 Use the full set of nucleotides shown in sequence on first page
 Each nucleotide: show as circled letter "P" for phosphate groups, sugars as simple pentagon shape,
nitrogenous bases as their first letter capitalized
 5' & 3' ends identified for all old and new strands
 Hydrogen bonding as shown in the first diagram (solid lines can be used)
 The replication fork should be shown between the third Thymine and Adenine on the DNA sequence shown
on page one. Show helicase at this fork moving "left" on your diagram.
 Show DNA replication occurring for both the leading and lagging strands including: RNA primase and it's
activity, DNA Polymerase III and it's activity and DNA Polymerase I and it's activity.
 Show direction of movement of every enzyme in every location
 Show and label two Okazaki fragments being joined by DNA Ligase on the lagging strand. Clearly label
which one is Okazaki fragment #1 and Okazaki fragment #2.
 Draw and label a variety of nucleoside triphosphates floating in the surrounding nucleoplasm
Diagram 3: Transcription
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Use all nucleotides, phosphates & sugars as a line, nitrogenous bases as l" letter capitalized
DNA sequence shown on page 1 is the strand that is transcribed (the noncoding / antisense / template strand)
First four bases represent the end of the promoter region
Bases 5 through 18 represent an exon
Bases 19 through 27 represent an intron
Bases 28 through 40 represent an exon
Show transcription in 3 stages
 Stage 1: the noncoding / antisense / template strand in the process of transcribing the mRNA
including the role ofhelicase and RNA polymerase
 Stage 2: show the unedited mRNA after transcription
 Stage 3: show the edited mRNA